Post-Translational Modification Analysis

Description / Workflow

Specific detection of post-translational modifications (PTMs) of proteins (phosphorylation, ubiquitination, etc.).

Applications

• Identification of new stimulus-dependent signaling pathways
• Detailed characterization of phosphorylation and ubiquitination networks

Requirements / Constraints

• Enrichment step required
• Sufficient sample quantity

Technologies

• Multiple Reaction Monitoring (MRM)
• Parallel Reaction Monitoring (PRM)
• Label-free
• Chemical labeling (TMT/iTRAQ)

Sites