Proteome Pole of Montpellier

Proteome Pole of Montpellier

Explore our platform and its various features in detail

PPM : Presentation

The Proteome Pole of Montpellier (PPM) is one of the platforms of the BioCampus Montpellier Research Support Unit and brings together the technological capabilities for proteomic analysis in Montpellier and the surrounding area.

Expertise in various fields of proteomics

We implement all types of medium‑ and high‑throughput proteomics approaches, and our expertise covers most current proteomics technologies, including:

  • Preparation of biological samples (purification, separation and prefractionation) for proteomic analyses
  • Large‑scale quantitative proteomics (with or without labeling)
  • Targeted quantitative proteomics, especially for biomarker validation
  • Study of post‑translational modifications such as phosphorylations and ubiquitinations
  • Study of protein–protein and protein–ligand interactions (e.g., AP‑MS)
  • Bioinformatics and biostatistics analysis of data with internationally referenced tools

Quality

The PPM platform, ISO 9001:2015 certified since 2015, ensures reliable, traceable, and internationally compliant proteomic analyses, while guaranteeing confidentiality and the valorization of results.

Member of the national infrastructure ProFI and IBiSA certified

PPM : Site FPP (IGF)

The Functional Proteomics Platform (FPP), specialized in high‑resolution mass spectrometry, offers cutting‑edge technologies and expertise dedicated to protein identification, protein quantification, differential analysis (with or without labeling), and protein partner discovery. The platform is also equipped with a mass spectrometer dedicated to structural proteomics, enabling the study of protein–protein and protein–ligand interactions as well as their structural dynamics under native conditions.

Our Expertise and Services

Our Team & Equipments

Role Name Phone Location
Scientific Directors Martial Séveno 92 17 IGF Sud, RDC 29
Séverine Chaumont-Dubel 92 17 IGF Sud, RDC 29
Technical Manager Serge Urbach Mass spectrometry analysis 92 17 IGF Sud, RDC 29
Bottom-up Proteomic Analysis Mathilde Decourcelle Mass spectrometry analysis 92 17 IGF Sud, RDC 29
Khadija El Koulali Biochemical sample preparation 92 17 IGF Sud, RDC 29
Structural Proteomics Activities Cherine Bechara Mass spectrometry analysis 93 45 IGF Sud, RDC 25
Tristan Girbau (CDD) Mass spectrometry analysis 93 45 IGF Nord, RDC 207c
Information System Oana Vigy Software engineering and data management 92 17 IGF Sud, RDC 29

When calling, please prefix the extension number with 04 34 35

Pricing

A quote will be provided upon request for any service inquiry

A quote will be provided upon request for any service inquiry

1 – Teams affiliated with a structure attached to one of the three BioCampus institutions (UM, INSERM, and CNRS).
2 – Other academic partners on site, included within the MUSE perimeter, and other off-site academic partners.

Service Prices (excl. tax) Description / Included
On-site Team1 Off-site Academic2 Private
Precipitation 4.01 5.00 10.00
  • TCA/Acetone precipitation
Gel 29.84 37.18 74.36
  • Gel loading
  • Migration
  • Fixation, staining, washing
In-gel digestion 24.64 30.70 61.41
  • Cutting, washing
  • Reduction/alkylation
  • Digestion (trypsin or other enzyme)
  • Extraction
In-gel digestion (other enzyme) 100.93 125.76 251.51
S-TRAP digestion 17.14 21.35 42.71
  • S-TRAP column (mini/micro)
  • Reduction/alkylation
  • Digestion (trypsin)
  • Washing
  • Elution
Liquid digestion ≤ 10 µg 4.41 5.50 11.00
  • Reduction/alkylation
  • Digestion
  • Desalting
Liquid digestion ≥ 10 µg 8.34 10.40 20.79
Double liquid digestion ≤ 10 µg 15.84 19.73 39.46
  • Reduction/alkylation
  • Digestion
  • Desalting
Double liquid digestion ≥ 10 µg 19.76 24.63 49.25
TMT2 150.75 187.84 375.69
  • Labeling of 2 samples
TMT6 535.97 667.81 1335.63
  • Labeling of 6 samples
TMT10 1184.24 1475.57 2951.14
  • Labeling of 10 samples
TMT16 1578.66 1967.01 3934.01
  • Labeling of 16 samples
Phospho enrichment 38.74 48.27 96.54
  • Enrichment using FeNTA and TiO₂
Fractionation 32.00 39.87 79.74
  • High pH reversed-phase fractionation (8 fractions)
Desalting 5.70 7.11 14.22
  • ZipTip / OMIX desalting
LC-MS/MS 100.24 111.10 333.30
  • Acquisition time on high-resolution system (per hour)
Biochemistry expertise 20.09 20.10 60.30
  • Sample preparation (proteins/peptides) – per hour
Data analysis 40.16 40.19 120.56
  • Bioinformatics processing – per hour

A quote will be provided upon request for any service inquiry

1- Teams affiliated with a structure attached to one of the three BioCampus institutions (UM, INSERM, and CNRS).
2- Other academic partners on site, included within the MUSE perimeter, and other off-site academic partners.

Service Prices (excl. tax) Description / Included
On-site Team1 Off-site Academic2 Private
Native analysis 1254.02 1441.25 1500
  • Dessalage et échange de tampon
  • Analyse (1/2 journée) par spectrométrie de masse (MS)
Native data analysis 85.92 104.80 200
  • 2 hours of bioinformatics (re)processing
HDX 4024.50 4627.44 8000
  • Hydrogen-deuterium exchange; digestion; HPLC-MS analysis (1 day)
HDX data analysis 569.41 653.93 1600
  • Reprocessing of HDX-MS results

FPP Site Contact

Scientific Directors :
Martial Séveno and Séverine Chaumont-Dubel

Technical Manager :
Serge Urbach

You can contact us via our contact form (by selecting the FPP site).

PPM : Site MSPP (IPSiM)

The Proteomic Mass Spectrometry Platform (MSPP) specializes in the characterization of post-translational modification events and signaling mechanisms involved in plant responses to various stresses, particularly quantitative changes in the phosphorylation of membrane proteins.

Our Expertise and Services

Equipments

Pricing

A quotation will be provided upon request for any service.

MSPP Site Contact

Scientific Director:
Christian Dubos

Technical Manager:
Valérie Rofidal

You can contact us via our contact form (by selecting the MSPP site).

PPM : Site PPC (IRMB)

The Clinical Proteomics Platform (PPC) aims to provide medical, biological, and technical expertise in clinical proteomics. The platform is dedicated to leveraging the latest technological advances in mass spectrometry and immunoassays for the discovery, validation, and application of biomarkers across a wide range of human diseases.

Our Expertise and Services

Pricing

A quotation will be provided upon request for any service.

Equipments

PPC Site Contact

Scientific Director:
Christophe Hirtz

Technical Manager:
Jérôme Vialaret

You can contact us via our contact form (by selecting the PPC site).

PPM : Organizational chart

Direction de la plateforme PPM

    Responsable Scientifique :

  • Martial SEVENO
    • (IR, CNRS, 40%)

    Responsable opérationnel :

  • Jerome VIALARET
    • (IE, CHU, 20%)
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Comité de Direction

  • Severine CHAUMONT-DUBEL
  • Christian DUBOS
  • Christophe HIRTZ
  • Alain MANGE
  • Valérie ROFIDAL
  • Martial SEVENO
  • Serge URBACH
  • Jerome VIALARET
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Comité d'orientation scientifique et technique

    Experts externes :

  • Ludovic BONHOMME
    • (GDEC, Clermont-Ferrand)
  • Pascale COSETTE
    • (Pissaro, Rouen)
  • Chiara GUERRERA
    • (PPN, Paris)
  • Christine SCHAEFFER
    • (LSMBO, Strasbourg)

    Experts locaux :

  • Jérôme DEJARDIN
    • (IGH, Montpellier)
  • Dominique HELMLINGER
    • (CRBM, Montpellier)
  • Hamza MAMERI
    • (IATE, Montpellier)
  • Jérôme MOREAUX
    • (IGH, Montpellier)
  • Bruno ROBERT
    • (IRCM, Montpellier)
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Correspondant

  • Responsable Management Qualité : Mathilde DECOURCELLE
  • Communication : Khadija EL KOULALI
  • Developpement durable : Khadija EL KOULALI
  • Communication : Oana VIGY
  • Informatique : Oana VIGY
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Protéomique fonctionnelle FPP - IGF

    Responsable Scientifique :

  • Severine CHAUMONT-DUBEL
    • (MC, UM, 15%)
  • Martial SEVENO
    • (IR, CNRS, 60%)

    Responsable Technique :

  • Serge URBACH
    • (IR, CNRS, 100%)

    Personnel plateau permanent :

  • Cherine BECHARA
    • (PU, UM, 10%)
  • Mathilde DECOURCELLE
    • (IE, CNRS, 80%)
  • Khadija EL KOULALI
    • (AI, CNRS, 100%)
  • Alain MANGE
    • (IR, UM, 30%)
  • Oana VIGY
    • (IE, CNRS, 90%)

    Personnel plateau CDD :

  • Tristan GIRBAU
    • (CDD IE, CNRS, 100%)
  • Florine LEIPP
    • (CDD IE, UM, 100%)
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Spectrométrie de masse protéomique MSPP - IPSiM

    Responsable Scientifique :

  • Christian DUBOS
    • (DR, INRAe, 20%)

    Responsable Technique :

  • Valérie ROFIDAL
    • (AI, INRAe, 100%)

    Personnel plateau permanent :

  • Nathalie BERGER
    • (IE, INRAe, 10%)
  • Vincent DEMOLOMBE
    • (T, INRAe, 100%)
  • Marc TAUZIN
    • (T, INRAe, 50%)
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Protéomique clinique PPC

    Responsable Scientifique :

  • Christophe HIRTZ
    • (PU, UM, 20%)

    Responsable Technique :

  • Jerome VIALARET
    • (IE, CHU, 80%)

    Personnel plateau permanent :

  • Alexandre DAVID
    • (DR, INSERM, 10%)
  • Constance DELABY
    • (MC, UM, 10%)

    Personnel plateau CDD :

  • Aurore ATTINA
    • (CDD IE, CHU, 100%)
  • Salomé COPPENS
    • (CDD IE, UM, 20%)
  • Maïssam EZZIANI
    • (CDD T, CHU, 100%)
  • Laura FICHTER
    • (CDD Thèse, UM, 20%)
  • Jana KINDERMANS
    • (CDD T, CHU, 100%)
  • Nicolas PRADEILLES
    • (CDD T, CHU, 100%)
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