Biological Sample Preparation for Proteomic Analyses

Description / Workflow

Handling of pre-analytical biochemical steps (protein separation and digestion, peptide enrichment and pre-fractionation, for example) for proteomics studies.

Applications

• Fractionation of complex protein mixtures by electrophoresis
• Enzymatic digestion of proteins (in-gel or in-solution)
• Protein enrichment (depletion of highly abundant proteins) or peptide enrichment (phosphopeptides)

Requirements / Constraints

Sample preparation requires a clean environment and equipment dedicated to proteomics analysis in order to minimize potential sources of contamination (keratins, polymers, etc.).

A sufficient amount of protein in the sample is required.

Each site is equipped with a biochemistry laboratory dedicated to this type of preparation (electrophoresis systems, laminar flow hoods, scanners, speed-vac, etc.).

Some equipment is available to collaborators wishing to prepare their own samples (subject to prior training by platform staff): please inquire.

Technologies

• SDS-PAGE 1D
• Digestion
• Fractionation
• Enrichment
• Depletion
• Desalting
• Chemical labeling (TMT/iTRAQ)

Sites