Quantitative Proteomics

Description / Workflow

Quantification is based on

• Label-free approaches: Samples are analyzed independently by LC-MS. This method requires multiple replicates and relies on the stability and reproducibility of the nanoLC-MS system.
• Isotopic labeling approaches: A mass tag containing heavy isotopes is introduced, either at the peptide level via isobaric labeling (TMT/iTRAQ) or at the protein level via metabolic labeling (SILAC). Samples can then be pooled and analyzed simultaneously.

Applications

• Basic and/or clinical research
• Study of proteome variations

Requirements / Constraints

• Selection of experimental design (label-free, SILAC, TMT, etc.) guided by sample type (cell cultures, tissues, etc.), complexity, and available material
• Sufficient number of experiments for statistical pre-processing
• High-resolution mass spectrometer

Technologies

• Label-free
• Chemical labeling (TMT/iTRAQ)
• Metabolic labeling (SILAC)

Sites