Support And Promote Health InfrastructuRes (SAPHIR)

Description

The Montpellier Proteome Core Facility participated in the Support And Promote Health InfrastructuRes (SAPHIR) project, led by UAR BioCampus, with the aim of acquiring and installing research and innovation equipment to enable the development of products and services in targeted fields (health, digital technology, and renewable energies).

Collaboration

As an example of the collaborations carried out within the SAPHIR project, the platform was approached by the team of Dr. Enzo Lalli (Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne) to conduct multiplexed quantitative proteomic studies aimed at characterising the proteome of adrenocortical carcinoma cell lines under various conditions of interest (gene of interest inactivation, presence of chemical inhibitors).

Equipment

The platform has been equipped with a complete LC-MS/MS configuration enabling multiplexed proteomic analyses:

• a high-resolution hybrid mass spectrometer Exploris 480, featuring Orbitrap technology
• a high-performance nano liquid chromatography system (UltiMate 3000 RSLC),
• a differential ion mobility interface (FAIMS Pro TM),
• a nitrogen generator.

HR

Two fixed-term staff recruitments were carried out in 2021. Ms El Koulali (CNRS Assistant Engineer, 100%) and Ms Ishimwe (Inserm Technician, 100%) took charge of biochemical sample preparation as well as part of the developments required to implement TMT chemical labelling multiplexing protocols, particularly when working with low amounts of material.

Date

2021-2022

Funding and support

The PPM project received funding of €835,814 from the Occitanie region through European funds under the REACT-EU recovery plan.

Publications

Ruggiero et al., 2023 (DOI: 10.1002/ijc.34526)

Platform

Exploris 480 (Thermo Fisher Scientific)